PANDAA Lassa Virus - Bio-Rad CFX96 ====================== 1. Download the .prcl and .pltd template files from https://www.aldatubio.com/qpcr-templates/ .prcl (Protocol File): Defines the temperature, time, and gradient settings for the PCR run. .pltd (Plate File): Contains well contents, fluorophores, and sample information. USING THE .prcl PROTOCOL FILE ====================== The .prcl file(s) provided by Aldatu Biosciences should be selected as the protocol when starting a User Defined run. USING THE .pltd PLATE FILE ====================== The .pltd is pre-loaded with the three assay targets. The PANDAA Lassa Virus - Qualification Panel - Plate Template - CFX96.pltd file is pre-loaded with the sample layout for the PANDAA LASV Qualification Panel. ANALYSIS SETTINGS ====================== It is not possible to save analysis settings in the template files, so when analysing the results: 1. Select "Target" as the analysis mode. 2. Baseline setting: baseline subtracted curve fit. 3. Export: Use "Custom Export" and export to Excel 2007 (*.xlsx) Target Name: PANDAA Lassa Virus (LASV) Threshold: Refer to PANDAA Lassa Virus instructions for use. Target Name: Internal Control (IC) Threshold: Auto Target Name: Sample Adequacy Control (SAC) Threshold: Auto