PANDAA Lassa Virus - Rotor-Gene Q
======================
For Rotor-Gene Q 5-Plex with 72-Well Rotor

1. Download the .ret template file from https://www.aldatubio.com/qpcr-templates/


RUN SETTINGS
======================
To prevent any run setting errors, avoid manually programming the Rotor-Gene Q and ensure that the template PANDAA Lassa Virus - RGQ Template.ret is used.

The Auto-Gain prompt that appears at the start of a new run should be set so that auto-gain optimisation is performed before the 1st acquisition. 

Do NOT perform gain optimisation at 60 °C at the beginning of the run.



ANALYSIS SETTINGS
======================
It is not possible to save analysis settings in the .ret template file, so when analysing the results: 

1. Dynamic Tube should be selected.
2. Slope Correct should be selected for consistent results across all runs.
3. Outlier Removal should be set at 5%.

Target Name:		PANDAA Lassa Virus (LASV)
Method:			Dynamic
Exclusion:		Extensive
Fluorescence Cutoff:	5%
Threshold:		Refer to PANDAA Lassa Virus instructions for use.

Target Name:		Internal Control (IC)
Method:			Dynamic
Exclusion:		None
Fluorescence Cutoff:	N/A
Threshold:		Auto

Target Name:		Sample Adequacy Control (SAC)
Method:			Dynamic
Exclusion:		None
Fluorescence Cutoff:	N/A
Threshold:		Auto